ELISA

Porcine CKMB (Creatine Kinase MB Isoenzyme) ELISA Kit

  • Catalog Number : E0022
  • Number : E0022
  • Size:
    Qty :
  • Price : Request 詢價
  • Stock : Request
  • Download DataSheet print

Introduction

This ELISA kit applies to the in vitro quantitative determination of Porcine CKMB concentrations in serum, plasma and other biological fluids.

General Information

Reactivity Porcine
Assay principle This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine CKMB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine CKMB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate
solution is added to each well. Only those wells that contain Porcine CKMB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine CKMB. You can calculate the concentration of Porcine CKMB in the samples by comparing the OD of the samples to the standard curve.
Sensitivity 0.14 ng/mL
Detection range 0.23-15 ng/mL
Sample type serum, plasma,cell lysates,tissue homogenates, cell culture supernatant and other biological fluids.
Storage instruction 4˚C or -20˚C for 6 months

CONTENTS and STORAGE

最近瀏覽產品

gotop link
客製化服務 產品 聯絡我們
0