Cofilin (Phospho Ser3) Rabbit pAb
- Catalog Number : AP0070
- Number : AP0070
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General Information
| Reactivity | Human, Mouse, Rat | ||||||||||||
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| Application | WB, IF, IHC, ELISA | ||||||||||||
| Host | Rabbit | ||||||||||||
| Clonality | Polyclonal | ||||||||||||
| Conjugate | Non-conjugated | ||||||||||||
| Isotype | IgG | ||||||||||||
| Immunogen | The antiserum was produced against synthesized peptide derived from human Cofilin around the phosphorylation site of Ser3. AA range:1-50 | ||||||||||||
| Molecular Weight | 19kD (Observed) | ||||||||||||
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||||||||||
| Storage instruction | -15°C to -25°C/1 year(Do not lower than -25°C) | ||||||||||||
| Research topic | >>Axon guidance>>Fc gamma R-mediated phagocytosis>>Regulation of actin cytoskeleton | ||||||||||||
| Alias | CFL1
CFL Cofilin-1 18 kDa phosphoprotein p18 Cofilin non-muscle isoform |
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| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200 | ||||||||||||
| Specificity | Phospho-Cofilin (S3) Polyclonal Antibody detects endogenous levels of Cofilin protein only when phosphorylated at S3.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):MAsGV | ||||||||||||
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | ||||||||||||
| Gene Name | CFL1 | ||||||||||||
| Protein Name | Cofilin-1 | ||||||||||||
| Database Link |
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| Background | cofilin 1(CFL1) Homo sapiens The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]. | ||||||||||||
| Function | Function:Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.,online information:Cofilin entry,PTM:Phosphorylated on Ser-3 in resting cells.,similarity:Belongs to the actin-binding proteins ADF family.,similarity:Contains 1 ADF-H domain.,subcellular location:Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.,tissue specificity:Widely distributed in various tissues. | ||||||||||||
| Cellular Localization | Nucleus matrix . Cytoplasm, cytoskeleton . Cell projection, ruffle membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, lamellipodium membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, lamellipodium . Cell projection, growth cone . Cell projection, axon . Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide. | ||||||||||||
| Tissue Expression | Widely distributed in various tissues. | ||||||||||||
| Validation Data |
Shi, Cuijuan, et al. "miR-29a/b/c function as invasion suppressors for gliomas by targeting CDC42 and predict the prognosis of patients." British Journal of Cancer 117.7 (2017): 1036. | ||||||||||||
Western Blot analysis of NIH-3T3 cells using Phospho-Cofilin (S3) Polyclonal Antibody | |||||||||||||
Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide. |
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